PHOSPHOROLYSIS OF OLIGORIBONUCLEOTIDES BY POLYNUCLEOTIDE PHOSPHORYLASE
نویسندگان
چکیده
منابع مشابه
Phosphorolysis of oligoribonucleotides by polynucleotide phosphorylase.
This reversible reaction has been demonstrated With enzyme preparations from Azotobacter trinelundii (1, 2) and Escherichia coli (3). In the forward direction the enzyme catalyzes the formation of polyribonucleotides similar in structural details to natural RNA1 (5-8). In the reverse reaction polyribonucleotides are phosphorolyzed to yield nucleoside diphosphates. The phosphorolysis of syntheti...
متن کاملThe effect of chain length on the phosphorolysis of oligonucleotides by polynucleotide phosphorylase.
The kinetics of phosphorolysis of compounds in the series (Ap),A (II = 2 to 8), p(Ap),A (n = 2 to 4), and (Up)I;cT, (II = 3 to 6) has been studied. K, and V,,, values were obtained for all compounds. K, decreases with increasing chain length and reaches a minimum value with compounds containing 5 phosphate residues. With the shorter compounds the dependence of K, on the nucleoside residues is a...
متن کاملPolynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
متن کاملStudies on Polynucleotides Synthesized by Polynucleotide Phosphorylase
The preparation of synthetic ribonucleic acid in which one of the nucleotide species is labeled with P32, makes possible studies on the distribution of the labeled nucleotide in the polynucleotide chain. Fig. 1 presents the structure of a polynucleotide in which labeled adenylic acid is randomly distributed. Hydrolysis of such a polymer with snake venom phosphodiesterase (Fig. 1A) will yield a ...
متن کاملStreptomyces coelicolor polynucleotide phosphorylase can polymerize nucleoside diphosphates under phosphorolysis conditions, with implications for the degradation of structured RNAs.
We have examined the ability of wild-type polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor and two mutant forms of the enzyme, N459D and C468A, to function in the polymerization of ADP and in the phosphorolysis of RNA substrates derived from the S. coelicolor rpsO-pnp operon. The wild-type enzyme was twice as active in polymerization as N459D and four times as active as C468A....
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70389-x